本研究通过头对头比较Vircell、Certest和Altona三种HDV RNA qRT-PCR检测试剂盒，揭示了其在定性诊断上高度一致（κ=0.988-1.000），但存在显著的定量偏倚（Vircell和Certest分别高估0.33和0.24 log10IU/mL）。同时，研究证实热休克（thermal shock）前处理虽能提升低病毒载量样本的 ...

COVID-19 is commonly diagnosed by quantitative fluorescence real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) (1, 2). The test is the sequential action of two enzymes–an ...

Polymerase chain reaction (PCR) tests have become routine because of the COVID-19 pandemic, with more than a million PCR tests being carried out daily in the United States alone. What are now commonly ...

With laboratory methods and diagnostics advancing apace, Francesca Lake considers whether PCR is likely to retain its crown as an essential lab method, or whether it could be replaced and consigned to ...