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RGFP966在绵羊体细胞核移植关键步骤中的应用对克隆胚胎发育的影响
本研究探讨了组蛋白去乙酰化酶3(HDAC3)选择性抑制剂(E)-N-(2-氨基-4-氟苯基)-3-(1-肉桂基-1H-吡唑-4-基)丙烯酰胺(RGFP966)在优化绵羊体细胞核移植(SCNT)关键步骤中的作用和机制。使用“18h + 4h”两步卵母细胞成熟方案,16μM的RGFP966将第一极体(PB1)的排出率 ...
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